Recombinant humaan sonisch egel/shh eiwit

SHH, also known as Sonic hedgehog protein, is one of three proteins in the mammalian signaling pathway family called hedgehog. It plays a key role in regulating vertebrate organogenesis, such as in the growth of digits on limbs and organization of the brain. It controls cell division of adult stem cells and has been implicated in development of some cancers. Recombinant human SHH protein, fused to His-tag at C-terminus was expressed in E. coli and purified by using conventional chromatography techniques.

Sonic hedgehog protein, HHG1, HLP3, HPE3, SMMCI, TPT, TPTPS, Sonic hedgehog protein HHG 1, HLP 3, Holoprosencephaly 3, HPE 3, MCOPCB5, SHH, SMMC I, SMMCI, Sonic Hedgehog (Drosophila) homolog, sonic hedgehog homolog (Drosophila), Sonic hedgehog homolog

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

20.7 kDa (183aa) confirmed by MALDI-TOF

Sonic Hedgehog, Shh, Sonic hedgehog protein, HHG1, HLP3, HPE3, SMMCI, TPT, TPTPS, Sonic hedgehog protein HHG 1, HLP 3, Holoprosencephaly 3, HPE 3, MCOPCB5, SHH, SMMC I, SMMCI, Sonic Hedgehog (Drosophila) homolog, sonic hedgehog homolog (Drosophila), Sonic hedgehog homolog, ATGP0575-10 µg, ATGP0575-20 µg, ATGP0575-50 µg, ATGP0575-100 µg, ATGP0575-250 µg, ATGP0575-500 µg, ATGP0575-1 mg, ATGP0575-10, ATGP0575-20, ATGP0575-50, ATGP0575-100, ATGP0575-250, ATGP0575-500, ATGP0575-1

Marigo V, et al. (1996) Proc Natl Acad Sci u S A. 93 (18) :9346-51; ; Kolpak A, et al. (2005) J Neurosci. 25 (13) :3432-41;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Cancer

NP_000184

Q15465

Human