Recombinant humaan Sorcine/SRI-eiwit

Sorcin, also known as SRI, is a 22-kDa calcium-binding protein initially identified in multidrug-resistant cells. SRI modulates excitation-contraction coupling in the heart and contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Recombinant human SRI protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Sorcin, 22 kDa protein, CP22, V19, SCN

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.1 kDa (221aa) confirmed by MALDI-TOF

SRI, Sorcin, 22 kDa protein, CP22, V19, SCN, ATGP0941-10 µg, ATGP0941-20 µg, ATGP0941-50 µg, ATGP0941-100 µg, ATGP0941-250 µg, ATGP0941-500 µg, ATGP0941-1 mg, ATGP0941-10, ATGP0941-20, ATGP0941-50, ATGP0941-100, ATGP0941-250, ATGP0941-500, ATGP0941-1

Landriscina M., et al. (2010) Cancer Res. 70 (16) :6577-86; ; Meyers MB., et al. (1995) J Biol Chem. 270 (44) :26411-8

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.2M NaCl, 1mM DTT, 0.1mM PMSF

Signal Transduction

NP_003121

P30626

Human

MGSSHHHHHH SSGLVPRGSH MGSMAYPGHP GAGGGYYPGG YGGAPGGPAF PGQTQDPLYG YFAAVAGQDG QIDADELQRC LTQSGIAGGY KPFNLETCRL MVSMLDRDMS GTMGFNEFKE LWAVLNGWRQ HFISFDTDRS GTVDPQELQK ALTTMGFRLS PQAVNSIAKR YSTNGKITFD DYIACCVKLR ALTDSFRRRD TAQQGVVNFP YDDFIQCVMS V