Recombinant humaan SORD-eiwit

SORD, also known as sorbitol dehydrogenase, is a member of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney and in the lens of the eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses. Recombinant human SORD protein, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 15 units/mg, and is defined as the amount of enzyme that catalyze the reduction 1.0 umole of D-fructose to D-sorbitol per minute at pH 7.5 at 37C.

SORD1, RDH, SDH, SORDD, XDH

E.coli

Human

Non-Tagged

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 15unit/mg, and is defined as the amount of enzyme that catalyze the reduction 1.0 umole of D-fructose to D-sorbitol per minute at pH 7.5 at 37C.

38.3 kDa (357aa)

SORD, SORD1, ATGP3293-10 µg, ATGP3293-20 µg, ATGP3293-50 µg, ATGP3293-100 µg, ATGP3293-250 µg, ATGP3293-500 µg, ATGP3293-1 mg, ATGP3293-10, ATGP3293-20, ATGP3293-50, ATGP3293-100, ATGP3293-250, ATGP3293-500, ATGP3293-1

Baker MA., et al. (2010) Proteomics. 10 (3) :482-95.; ; Sorger D., et al. (2009) Nucl Med Biol. 36 (1) :17-27.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol, 1mM DTT

Enzymes & Proteases

NP_003095.1

Q00796

Human

MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP