Recombinant humaan SPA17-eiwit

SPA17 is a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. Recombinant human SPA17 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sperm surface protein Sp17, CT22, SP17, SP17-1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

19.8 kDa (174aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

SPA17, Sperm surface protein Sp17, CT22, SP17, SP17-1, ATGP2045-10 µg, ATGP2045-20 µg, ATGP2045-50 µg, ATGP2045-100 µg, ATGP2045-250 µg, ATGP2045-500 µg, ATGP2045-1 mg, ATGP2045-10, ATGP2045-20, ATGP2045-50, ATGP2045-100, ATGP2045-250, ATGP2045-500, ATGP2045-1

Buchli R., et al. (2002) Biochim. Biophys. Acta. 1578:29-42; ; Li Z., et al. (2007) Int. J. Cancer. 121:1507-1511;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Cancer

NP_059121

Q15506

Human

MGSSHHHHHH SSGLVPRGSH MGSMSIPFSN THYRIPQGFG NLLEGLTREI LREQPDNIPA FAAAYFESLL EKREKTNFDP AEWGSKVEDR FYNNHAFEEQ EPPEKSDPKQ EESQISGKEE ETSVTILDSS EEDKEKEEVA AVKIQAAFRG HIAREEAKKM KTNSLQNEEK EENK