Recombinant humaan SPARC-eiwit

SPARC, also known as osteonectin, is a member of secreted matricellular proteins. It is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. This protein regulates cell growth through interactions with the extracellular matrix and cytokines and binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. It functions as modulating cell-cell and cell-matrix interactions and its de-adhesive and growth inhibitory properties in non-transformed cell. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. A correlation between SPARC over-expression and ampullary cancers and chronic pancreatitis has been found. It also shows affinity for collagen in addition to bone mineral calcium. Recent studies have also demonstrated a role for SPARC in sensitizing therapy-resistant cancers. Notably, it is linked to human obesity. Recombinant human SPARC, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

SPARC, Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine, OI17

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

33.8 kDa (295aa)

SPARC, ATGP3902-10 µg, ATGP3902-20 µg, ATGP3902-50 µg, ATGP3902-100 µg, ATGP3902-250 µg, ATGP3902-500 µg, ATGP3902-1 mg, ATGP3902-10, ATGP3902-20, ATGP3902-50, ATGP3902-100, ATGP3902-250, ATGP3902-500, ATGP3902-1

McDonald LT., et al. (2018) Am J Physiol Heart Circ Physiol. 315 (1) :H92-H100.; ; Wang B., et al. (2018) J Biol Regul Homeost Agents. 32:1525-1531.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cell Cycle

NP_003109

P09486

Human

Ala

APQQEAL PDETEVVEET VAEVTEVSVG ANPVQVEVGE FDDGAEETEE EVVAENPCQN HHCKHGKVCE LDENNTPMCV CQDPTSCPAP IGEFEKVCSN DNKTFDSSCH FFATKCTLEG TKKGHKLHLD YIGPCKYIPP CLDSELTEFP LRMRDWLKNV LVTLYERDED NNLLTEKQKL RVKKIHENEK RLEAGDHPVE LLARDFEKNY NMYIFPVHWQ FGQLDQHPID GYLSHTELAP LRAPLIPMEH CTTRFFETCD LDNDKYIALD EWAGCFGIKQ KDIDKDLVI