Recombinant humaan SPECC1-eiwit

SPECC1, also known as Cytospin-B, belongs to the cytospin-A family. This protein is involved in cytokinesis and spindle organization. It may play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration. Recombinant human SPECC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytospin-B, CYTSB, HCMOGT-1, HCMOGT1, NSP

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

37.7 kDa (327aa) confirmed by MALDI-TOF

SPECC1, Cytospin-B, CYTSB, HCMOGT-1, HCMOGT1, NSP, ATGP2733-10 µg, ATGP2733-20 µg, ATGP2733-50 µg, ATGP2733-100 µg, ATGP2733-250 µg, ATGP2733-500 µg, ATGP2733-1 mg, ATGP2733-10, ATGP2733-20, ATGP2733-50, ATGP2733-100, ATGP2733-250, ATGP2733-500, ATGP2733-1

Sang N. et al. (2004) Oncogene. 23:9438-9446.; ; D'Agostino L. et al. (2008) Cell Cycle. 7:1810-1827

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT, 0.1M NaCl

Cell Cycle

NP_690868

Q5M775

Human

MGSSHHHHHH SSGLVPRGSH MGSTQEGKII ELEQKCTGIL EQGRFEREKL LNIQQQLTCS LRKVEEENQG ALEMIKRLKE ENEKLNEFLE LERHNNNMMA KTLEECRVTL EGLKMENGSL KSHLQGEKQK ATEASAVEQT AESCEVQEML KVARAEKDLL ELSCNELRQE LLKANGEIKH VSSLLAKVEK DYSYLKEICD HQAEQLSRTS LKLQEKASES DAEIKDMKET IFELEDQVEQ HRAVKLHNNQ LISELESSVI KLEEQKSDLE RQLKTLTKQM KEETEEWRRF QADLQTAVVV ANDIKCEAQQ ELRTVKRKLL EEEEKNA