Recombinant humaan Spermine synthase/SMS-eiwit

SMS (Spermine synthase) belongs to the spermidine/spermine synthase family. It is an enzyme that converts spermidine into spermine. This enzyme is required for normal viability, growth and fertility involved in polyamine metabolism. Defects in SMS are the cause of Snyder-Robinson syndrome (SRS), also known as X-linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive. Recombinant human SMS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Spermine synthase, MRSR, SPMSY, SpS, SRS

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

43.8 kDa (390aa) confirmed by MALDI-TOF

SMS, Spermine synthase, MRSR, SPMSY, SpS, SRS, ATGP1358-10 µg, ATGP1358-20 µg, ATGP1358-50 µg, ATGP1358-100 µg, ATGP1358-250 µg, ATGP1358-500 µg, ATGP1358-1 mg, ATGP1358-10, ATGP1358-20, ATGP1358-50, ATGP1358-100, ATGP1358-250, ATGP1358-500, ATGP1358-1

Cason A.L., et al. (2003) Eur. J. Hum. Genet. 11:937-944; ; Sowell J, et al. (2011) Clin Chim Acta. 18; ;412 (7-8) :655-60.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Metabolism

NP_004586.2

P52788

Human

MAAARH STLDFMLGAK ADGETILKGL QSIFQEQGMA ESVHTWQDHG YLATYTNKNG SFANLRIYPH GLVLLDLQSY DGDAQGKEEI DSILNKVEER MKELSQDSTG RVKRLPPIVR GGAIDRYWPT ADGRLVEYDI DEVVYDEDSP YQNIKILHSK QFGNILILSG DVNLAESDLA YTRAIMGSGK EDYTGKDVLI LGGGDGGILC EIVKLKPKMV TMVEIDQMVI DGCKKYMRKT CGDVLDNLKG DCYQVLIEDC IPVLKRYAKE GREFDYVIND LTAVPISTSP EEDSTWEFLR LILDLSMKVL KQDGKYFTQG NCVNLTEALS LYEEQLGRLY CPVEFSKEIV CVPSYLELWV FYTVWKKAKP