Recombinant humaan SPIN1-eiwit

SPIN1 belongs to the SPIN/STSY family. It is expressed in various tissues and cells, is localized to nucleus. This protein may play a role in cell-cycle regulation during the transition from gamete to embryo. Recombinant human SPIN1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Spindlin-1, Spin

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

32kDa (285aa) confirmed by MALDI-TOF

SPIN1, Spindlin-1, Spin, ATGP1589-10 µg, ATGP1589-20 µg, ATGP1589-50 µg, ATGP1589-100 µg, ATGP1589-250 µg, ATGP1589-500 µg, ATGP1589-1 mg, ATGP1589-10, ATGP1589-20, ATGP1589-50, ATGP1589-100, ATGP1589-250, ATGP1589-500, ATGP1589-1

Zhang, P.et al. (2008) J. Cell. Physiol. 217 (2), 400-408; ; Yuan, H., et al. (2008) Gene 410 (1), 67-74

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.15M NaCl

Cell Cycle

NP_006708

Q9Y657

Human

MGSSHHHHHH SSGLVPRGSH MGSMKTPFGK TPGQRSRADA GHAGVSANMM KKRTSHKKHR SSVGPSKPVS QPRRNIVGCR IQHGWKEGNG PVTQWKGTVL DQVPVNPSLY LIKYDGFDCV YGLELNKDER VSALEVLPDR VATSRISDAH LADTMIGKAV EHMFETEDGS KDEWRGMVLA RAPVMNTWFY ITYEKDPVLY MYQLLDDYKE GDLRIMPDSN DSPPAEREPG EVVDSLVGKQ VEYAKEDGSK RTGMVIHQVE AKPSVYFIKF DDDFHIYVYD LVKTS