Recombinant humaan SPINK1-eiwit

SPINK1 is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. Recombinant human SPINK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Serine peptidase inhibitor Kazal type 1, Serine peptidase inhibitor, Kazal type 1, PCTT, PSTI, Spink3, TATI, TCP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

8.6 kDa (79aa) confirmed by MALDI-TOF

SPINK1, Serine peptidase inhibitor Kazal type 1, Serine peptidase inhibitor, Kazal type 1, PCTT, PSTI, Spink3, TATI, TCP, ATGP2197-10 µg, ATGP2197-20 µg, ATGP2197-50 µg, ATGP2197-100 µg, ATGP2197-250 µg, ATGP2197-500 µg, ATGP2197-1 mg, ATGP2197-10, ATGP2197-20, ATGP2197-50, ATGP2197-100, ATGP2197-250, ATGP2197-500, ATGP2197-1

Witt H., et al. (2000) Nat. Genet. 25:213-216; ; Hassan Z., et al. (2002) Am. J. Hum. Genet. 71:964-968;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Enzymes & Proteases

NP_003113

P00995

Human

DSLGREA KCYNELNGCT KIYDPVCGTD GNTYPNECVL CFENRKRQTS ILIQKSGPC