Recombinant humaan SPOP-eiwit

SPOP (speckle-type POZ protein), also known as TEF2, is a member of the Tdpoz family containing one N-terminal MATH (Meprin and TRAF homology) domain and one C-terminal BTB/POZ domain. This protein inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex. Through an interaction with CuL-3, SPOP is involved in ubiquitinylation and protein degradation. Recombinant human SPOP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Speckle-type POZ protein, TEF2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

44.3 kDa (394aa) confirmed by MALDI-TOF

SPOP, Speckle-type POZ protein, TEF2, ATGP1009-10 µg, ATGP1009-20 µg, ATGP1009-50 µg, ATGP1009-100 µg, ATGP1009-250 µg, ATGP1009-500 µg, ATGP1009-1 mg, ATGP1009-10, ATGP1009-20, ATGP1009-50, ATGP1009-100, ATGP1009-250, ATGP1009-500, ATGP1009-1

Kwon J.E., et al. (2006) J. Biol. Chem. 281:12664-12672; ; Furukawa M., et al. (2003) Nat. Cell Biol. 5:1001-1007

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 0.2M NaCl, 2mM EDTA

Ubiquitination

NP_001007231

O43791

Human

MGSSHHHHHH SSGLVPRGSH MSRVPSPPPP AEMSSGPVAE SWCYTQIKVV KFSYMWTINN FSFCREEMGE VIKSSTFSSG ANDKLKWCLR VNPKGLDEES KDYLSLYLLL VSCPKSEVRA KFKFSILNAK GEETKAMESQ RAYRFVQGKD WGFKKFIRRD FLLDEANGLL PDDKLTLFCE VSVVQDSVNI SGQNTMNMVK VPECRLADEL GGLWENSRFT DCCLCVAGQE FQAHKAILAA RSPVFSAMFE HEMEESKKNR VEINDVEPEV FKEMMCFIYT GKAPNLDKMA DDLLAAADKY ALERLKVMCE DALCSNLSVE NAAEILILAD LHSADQLKTQ AVDFINYHAS DVLETSGWKS MVVSHPHLVA EAYRSLASAQ CPFLGPPRKR LKQS