Recombinant humaan SPRED1-eiwit

SPRED1 is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. This protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS) . Recombinant human SPRED1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Sprouty-related EVH1 domain-containing protein 1, Sprouty-related, EVH1 domain-containing protein 1, hSpred1, NFLS, spred-1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

52.9 kDa (467aa)

SPRED1, Sprouty-related EVH1 domain-containing protein 1, Sprouty-related, EVH1 domain-containing protein 1, hSpred1, NFLS, spred-1, ATGP2853-10 µg, ATGP2853-20 µg, ATGP2853-50 µg, ATGP2853-100 µg, ATGP2853-250 µg, ATGP2853-500 µg, ATGP2853-1 mg, ATGP2853-10, ATGP2853-20, ATGP2853-50, ATGP2853-100, ATGP2853-250, ATGP2853-500, ATGP2853-1

Nonami A., et al. (2005) Genes Cells 10:887-895.; ; King J.A.J., et al. (2005) Biochem. J. 388:445-454.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Signal Transduction

NP_689807

Q7Z699

Human

MSEETAT SDNDNSYARV RAVVMTRDDS SGGWLPLGGS GLSSVTVFKV PHQEENGCAD FFIRGERLRD KMVVLECMLK KDLIYNKVTP TFHHWKIDDK KFGLTFQSPA DARAFDRGIR RAIEDISQGC PESKNEAEGA DDLQANEEDS SSSLVKDHLF QQETVVTSEP YRSSNIRPSP FEDLNARRVY MQSQANQITF GQPGLDIQSR SMEYVQRQIS KECGSLKSQN RVPLKSIRHV SFQDEDEIVR INPRDILIRR YADYRHPDMW KNDLERDDAD SSIQFSKPDS KKSDYLYSCG DETKLSSPKD SVVFKTQPSS LKIKKSKRRK EDGERSRCVY CQERFNHEEN VRGKCQDAPD PIKRCIYQVS CMLCAESMLY HCMSDSEGDF SDPCSCDTSD DKFCLRWLAL VALSFIVPCM CCYVPLRMCH RCGEACGCCG GKHKAAG