Recombinant humaan SPSB1-eiwit

SPRY domain-containing SOCS box protein 1 (SPSB1), also known as SSB1, is a member of the SOCS box protein subfamily. This protein contains a central SPRY domain and a C-terminal SOCS box. Although some of the SOCS protein subfamilies function as adaptors for a large family of ubiquitin-protein isopeptide ligases to regulate certain signaling pathways, the function of the SSB subfamily remains to be determined. SPSB1 may play an important role in enhancing the HGF-induced Erk-Elk-1-SRE pathway. Over expression of SPSB1 exhibited no effect on the basal level or epidermal growth factor-induced SRE-luciferase activity. Recombinant human SPSB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

SPRY domain-containing SOCS box protein 1, SSB-1, SSB1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

26.1 kDa (231aa) confirmed by MALDI-TOF

SPSB1, SPRY domain-containing SOCS box protein 1, SSB-1, SSB1, ATGP1054-10 µg, ATGP1054-20 µg, ATGP1054-50 µg, ATGP1054-100 µg, ATGP1054-250 µg, ATGP1054-500 µg, ATGP1054-1 mg, ATGP1054-10, ATGP1054-20, ATGP1054-50, ATGP1054-100, ATGP1054-250, ATGP1054-500, ATGP1054-1

Woo J S., et al. (2006) Equb. 25 (6) :1353-63.; ; Wang D., et al. (2005) J Biol Chem. 280 (16) :16393-401.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Ubiquitination

NP_079382

Q96BD6

Human

QELQGLDYC KPTRLDLLLD MPPVSYDVQL LHSWNNNDRS LNVFVKEDDK LIFHRHPVAQ STDAIRGKVG YTRGLHVWQI TWAMRQRGTH AVVGVATADA PLHSVGYTTL VGNNHESWGW DLGRNRLYHD GKNQPSKTYP AFLEPDETFI VPDSFLVALD MDDGTLSFIV DGQYMGVAFR GLKGKKLYPV VSAVWGHCEI RMRYLNGLDP E