Recombinant humaan SRP14-eiwit

SRP14 is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum. The Alu domain of SRP comprises the heterodimer of the SRP9 and SRP14 proteins, which are bound to the 5' and 3' terminal sequences of SRP RNA. SRP9/14 binding may be crucial to the transcription, maturation, nucleolus localization and transport of SRP RNA. Recombinant human SRP14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Signal recognition particle 14 kDa protein, Signal recognition particle 14 kDa protein, DKFZp468D2027

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

17.1 kDa (160aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

SRP14, Signal recognition particle 14 kDa protein, Signal recognition particle 14 kDa protein, DKFZp468D2027, ATGP0799-10 µg, ATGP0799-20 µg, ATGP0799-50 µg, ATGP0799-100 µg, ATGP0799-250 µg, ATGP0799-500 µg, ATGP0799-1 mg, ATGP0799-10, ATGP0799-20, ATGP0799-50, ATGP0799-100, ATGP0799-250, ATGP0799-500, ATGP0799-1

Chang D.Y., et al. (1994), Mol. Cell. Biol, 14:3949-3959.; ; Hsu K., et al. (1995) . J. Biol. Chem., 270:10179-10186

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Signal Transduction

NP_003125.2

P37108

Human

MGSSHHHHHH SSGLVPRGSH MGSHMVLLES EQFLTELTRL FQKCRTSGSV YITLKKYDGR TKPIPKKGTV EGFEPADNKC LLRATDGKKK ISTVVSSKEV NKFQMAYSNL LRANMDGLKK RDKKNKTKKT KAAAAAAAAA PAAAATAATT AATTAATAAQ