Recombinant humaan SRR-eiwit

SRR, also known as serine racemase, is an enzyme which generates D-serine from L-serine. D-serine acts as a neuronal signaling molecule by activating NMDA receptors in the brain. Mammalian serine racemase is a pyridoxal 5'-phosphate dependent enzyme that catalyzes both the racemization of L-serine to D-serine and also the elimination of water from L-serine, generating pyruvate and ammonia. The enzyme is physiologically stimulated by divalent cations (e. g., magnesium) and is allosterically activated by the magnesium/ATP complex. Recombinant human SRR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Serine racemase, ILV1, ISO1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

39.1 kDa (364aa) confirmed by MALDI-TOF

SRR, Serine racemase, ILV1, ISO1, ATGP1369-10 µg, ATGP1369-20 µg, ATGP1369-50 µg, ATGP1369-100 µg, ATGP1369-250 µg, ATGP1369-500 µg, ATGP1369-1 mg, ATGP1369-10, ATGP1369-20, ATGP1369-50, ATGP1369-100, ATGP1369-250, ATGP1369-500, ATGP1369-1

De Miranda J., et al. (2000) Gene. 256:183-188; ; Smith M.A., et al. (2010) J. Biol. Chem. 285:12873-12881

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Neuroscience

NP_068766

Q9GZT4

Human

MGSSHHHHHH SSGLVPRGSH MGSHMCAQYC ISFADVEKAH INIRDSIHLT PVLTSSILNQ LTGRNLFFKC ELFQKTGSFK IRGALNAVRS LVPDALERKP KAVVTHSSGN HGQALTYAAK LEGIPAYIVV PQTAPDCKKL AIQAYGASIV YCEPSDESRE NVAKRVTEET EGIMVHPNQE PAVIAGQGTI ALEVLNQVPL VDALVVPVGG GGMLAGIAIT VKALKPSVKV YAAEPSNADD CYQSKLKGKL MPNLYPPETI ADGVKSSIGL NTWPIIRDLV DDIFTVTEDE IKCATQLVWE RMKLLIEPTA GVGVAAVLSQ HFQTVSPEVK NICIVLSGGN VDLTSSITWV KQAERPASYQ SVSV