Recombinant humaan SRSF1-eiwit

SRSF1, also known as Serine/arginine-rich splicing factor 1, is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. This protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. In addition to being involved in the splicing process, it also mediates post-splicing activities, such as mRNA nuclear export and translation. Recombinant human SRSF1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Serine/arginine-rich splicing factor 1, SRSF1, ASF, SF2, SF2p33, SFRS1, SRp30a

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford Assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

28.5 kDa (254aa)

SRSF1, Serine/arginine-rich splicing factor 1, SRSF1, ASF, SF2, SF2p33, SFRS1, SRp30a, ATGP3558-10 µg, ATGP3558-20 µg, ATGP3558-50 µg, ATGP3558-100 µg, ATGP3558-250 µg, ATGP3558-500 µg, ATGP3558-1 mg, ATGP3558-10, ATGP3558-20, ATGP3558-50, ATGP3558-100, ATGP3558-250, ATGP3558-500, ATGP3558-1

Xue F., et al. (2015) PLoS One. 10: e0115354.; ; Goncalves V., et al. (2014) RNA. 20:474-482.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM KCl, 1mM DTT, 0.2mM EDTA, 40% glycerol

Epigenomics (Transcription & Translation)

NP_008855

Q07955

Human

Met1

MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFPR SGRGTGRGGG GGGGGGAPRG RYGPPSRRSE NRVVVSGLPP SGSWQDLKDH MREAGDVCYA DVYRDGTGVV EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP SYGRSRSRSR SRSRSRSRSN SRSRSYSPRR SRGSPRYSPR HSRSRSRTHH HHHH