Recombinant humaan SSR1-eiwit

The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. Recombinant human SSR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Translocon-associated protein subunit alpha precursor, Signal sequence receptor, alpha, TRAPA

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.1 kDa (209aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

SSR1, Translocon-associated protein subunit alpha precursor, Signal sequence receptor, alpha, TRAPA, ATGP2432-10 µg, ATGP2432-20 µg, ATGP2432-50 µg, ATGP2432-100 µg, ATGP2432-250 µg, ATGP2432-500 µg, ATGP2432-1 mg, ATGP2432-10, ATGP2432-20, ATGP2432-50, ATGP2432-100, ATGP2432-250, ATGP2432-500, ATGP2432-1

Hartmann E., Prehn S. (1994), FEBS Lett. 349:324-326; ; Hirama T., Miller C.W., et al. (1999), FEBS Lett. 455:223-227

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Signal Transduction

NP_003135

P43307

Human

MGSSHHHHHH SSGLVPRGSH MGSRGGPRGL LAVAQDLTED EETVEDSIIE DEDDEAEVEE DEPTDLVEDK EEEDVSGEPE ASPSADTTIL FVKGEDFPAN NIVKFLVGFT NKGTEDFIVE SLDASFRYPQ DYQFYIQNFT ALPLNTVVPP QRQATFEYSF IPAEPMGGRP FGLVINLNYK DLNGNVFQDA VFNQTVTVIE REDGLDGET