Recombinant humaan ST6GAL1-eiwit

ST6GAL1, also known as beta-galactoside alpha-2, 6-sialyltransferase 1, is a type II membrane protein localized in the trans-Golgi network and catalyzes 2, 6-sialylation of Gal beta 1, 4-GlcNAc structures on N-glycans. It is highly expressed in the liver and also expressed in most other tissues to some extent. Its deficiency causes abnormalities in B cell immunoreactivity. Recombinant human ST6GAL1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Beta-galactoside alpha-2,6-sialyltransferase 1, ST6GAL1, SIAT1, ST6GalI, ST6N

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

44.6 kDa (389aa)

ST6GAL1, Beta-galactoside alpha-2,6-sialyltransferase 1, ST6GAL1, SIAT1, ST6GalI, ST6N, ATGP3338-10 µg, ATGP3338-20 µg, ATGP3338-50 µg, ATGP3338-100 µg, ATGP3338-250 µg, ATGP3338-500 µg, ATGP3338-1 mg, ATGP3338-10, ATGP3338-20, ATGP3338-50, ATGP3338-100, ATGP3338-250, ATGP3338-500, ATGP3338-1

Kuhn B., et al. (2013) Acta Crystallogr D Biol Crystallogr. 69:1826-1838.; ; Wu ZL., et al. (2011) Glycobiology. 21:727-733.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

NP_775323

P15907

Human

Ala

KEKKKGS YYDSFKLQTK EFQVLKSLGK LAMGSDSQSV SSSSTQDPHR GRQTLGSLRG LAKAKPEASF QVWNKDSSSK NLIPRLQKIW KNYLSMNKYK VSYKGPGPGI KFSAEALRCH LRDHVNVSMV EVTDFPFNTS EWEGYLPKES IRTKAGPWGR CAVVSSAGSL KSSQLGREID DHDAVLRFNG APTANFQQDV GTKTTIRLMN SQLVTTEKRF LKDSLYNEGI LIVWDPSVYH SDIPKWYQNP DYNFFNNYKT YRKLHPNQPF YILKPQMPWE LWDILQEISP EEIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC YYYQKFFDSA CTMGAYHPLL YEKNLVKHLN QGTDEDIYLL GKATLPGFRT IHC