Recombinant humaan STAR-eiwit

STAR, also known as steroidogenic acute regulatory protein, appears to mediate the rapid increase in pregnenolone synthesis stimulated by tropic hormones. It increases pregnenolone synthesis more than four-fold and a major STAR transcript of 1. 6 kb is found in ovary and testis. During ongoing growth and differentiation of the follicle of the ovary, the immunoreactivity of it tends to shift from the granulosa cells of early antral follicles to the theca cell layers in the adult. Recombinant human STAR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Steroidogenic acute regulatory protein mitochondrial, Steroidogenic acute regulatory protein, mitochondrial

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.1 kDa (243aa) confirmed by MALDI-TOF

STAR, Steroidogenic acute regulatory protein mitochondrial, Steroidogenic acute regulatory protein, mitochondrial, ATGP1292-10 µg, ATGP1292-20 µg, ATGP1292-50 µg, ATGP1292-100 µg, ATGP1292-250 µg, ATGP1292-500 µg, ATGP1292-1 mg, ATGP1292-10, ATGP1292-20, ATGP1292-50, ATGP1292-100, ATGP1292-250, ATGP1292-500, ATGP1292-1

Borthwick F. et al. (2009) FEBS Lett. 583:1147-1153.; ; Mikhaylova I.V. et al. (2008) J. Endocrinol.199: 435-444.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT

Cancer

NP_000340.2

P49675

Human

EETLYSDQE LAYLQQGEEA MQKALGILSN QEGWKKESQQ DNGDKVMSKV VPDVGKVFRL EVVVDQPMER LYEELVERME AMGEWNPNVK EIKVLQKIGK DTFITHELAA EAAGNLVGPR DFVSVRCAKR RGSTCVLAGM ATDFGNMPEQ KGVIRAEHGP TCMVLHPLAG SPSKTKLTWL LSIDLKGWLP KSIINQVLSQ TQVDFANHLR KRLESHPASE ARC