Recombinant humaan Stathmin-1/STMN1-eiwit

Stathmin is a ubiquitous phosphoprotein proposed to play a general role as an intracellular relay integrating diverse regulatory signals of the cellular environment. Also, Stathmin protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. The role of Stathmin in regulation of the cell cycle causes it to be an oncoprotein (Oncoprotein 18, op18) . When Stathmin is mutated and not functioning properly, this protein can cause uncontrolled cell proliferation. Recombinant human Stathmin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

STMN1, Stathmin1, Stathmin, SMN, Protein Pr22, Prosolin, PR22, PP19, Phosphoprotein p19, pp17, Phosphoprotein 19, p19, p18, OP18, Oncoprotein 18, Metablastin, Leukemia associated phosphoprotein p18, LAP18, Lag

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

19.4 kDa (169aa) confirmed by MALDI-TOF

Stathmin, ATGP0279-10 µg, ATGP0279-20 µg, ATGP0279-50 µg, ATGP0279-100 µg, ATGP0279-250 µg, ATGP0279-500 µg, ATGP0279-1 mg, ATGP0279-10, ATGP0279-20, ATGP0279-50, ATGP0279-100, ATGP0279-250, ATGP0279-500, ATGP0279-1

Maucuer A., et al. (1990) . FEBS Lett. 264 (2) :275-8; ; Sobel A., et al. (1991) . Trends Biochem Sci. 16 (8) :301-5 ; ; Steinmetz MO., et al. (2007) . J Struct Biol. 158 (2) :137-47;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Cell Cycle

NP_981944

P16949

Human

MASSDIQVKE LEKRASGQAF ELILSPRSKE SVPEFPLSPP KKKDLSLEEI QKKLEAAEER RKSHEAEVLK QLAEKREHEK EVLQKAIEEN NNFSKMAEEK LTHKMEANKE NREAQMAAKL ERLREKDKHI EEVRKNKESK DPADETEAD