Recombinant humaan STK16-eiwit

STK16, also known as serine/threonine-protein kinase 16, is a membrane-associated protein kinase that phosphorylates on serine and threonine residues. STK16 may be involved in secretory vesicle trafficking or intracellular signaling. Also this protein may have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. It able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins. Recombinant human STK16 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Serine/threonine-protein kinase 16, KRCT, MPSK, PKL12, TSF1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.2 kDa (329aa) confirmed by MALDI-TOF

STK16, Serine/threonine-protein kinase 16, KRCT, MPSK, PKL12, TSF1, ATGP1567-10 µg, ATGP1567-20 µg, ATGP1567-50 µg, ATGP1567-100 µg, ATGP1567-250 µg, ATGP1567-500 µg, ATGP1567-1 mg, ATGP1567-10, ATGP1567-20, ATGP1567-50, ATGP1567-100, ATGP1567-250, ATGP1567-500, ATGP1567-1

Berson A.E., et al. (1999) Biochem. Biophys. Res. Commun. 259:533-538; ; Eswaran J., et al. (2009) Structure. 16:115-124

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Signal Transduction

NP_001008910

O75716

Human

MGSSHHHHHH SSGLVPRGSH MGSHMGHALC VCSRGTVIID NKRYLFIQKL GEGGFSYVDL VEGLHDGHFY ALKRILCHEQ QDREEAQREA DMHRLFNHPN ILRLVAYCLR ERGAKHEAWL LLPFFKRGTL WNEIERLKDK GNFLTEDQIL WLLLGICRGL EAIHAKGYAH RDLKPTNILL GDEGQPVLMD LGSMNQACIH VEGSRQALTL QDWAAQRCTI SYRAPELFSV QSHCVIDERT DVWSLGCVLY AMMFGEGPYD MVFQKGDSVA LAVQNQLSIP QSPRHSSALR QLLNSMMTVD PHQRPHIPLL LSQLEALQPP APGQHTTQI