Recombinant humaan stollingsfactor III/weefselfactoreiwit

Tissue factor, also known as F3 or CD142, is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Recombinant human F3 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tissue factor, CD142, TF, TFA

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.9 kDa (228aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Coagulation Factor III, Tissue Factor, Tissue factor, CD142, TF, TFA, ATGP1683-10 µg, ATGP1683-20 µg, ATGP1683-50 µg, ATGP1683-100 µg, ATGP1683-250 µg, ATGP1683-500 µg, ATGP1683-1 mg, ATGP1683-10, ATGP1683-20, ATGP1683-50, ATGP1683-100, ATGP1683-250, ATGP1683-500, ATGP1683-1

Bogdanov V., et al. (2003) Nat. Med. 9:458-462; ; Mackman N., et al. (1989) Biochemistry. 28:1755-1762

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Cardiovascular

NP_001984

P13726

Human