Recombinant humaan STYX-eiwit

STYX, also known as serine/threonine/tyrosine-interacting protein, belongs to the protein-tyrosine phosphatase family. It contains a Gly residue instead of a conserved Cys residue in the dsPTPase catalytic loop which renders it catalytically inactive as a phosphatase. The binding pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. It seems to play a role in spermiogenesis. Recombinant human STYX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Serine/threonine/tyrosine-interacting protein, FLJ42934

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

28.0 kDa (247aa) confirmed by MALDI-TOF

STYX, Serine/threonine/tyrosine-interacting protein, FLJ42934, ATGP1443-10 µg, ATGP1443-20 µg, ATGP1443-50 µg, ATGP1443-100 µg, ATGP1443-250 µg, ATGP1443-500 µg, ATGP1443-1 mg, ATGP1443-10, ATGP1443-20, ATGP1443-50, ATGP1443-100, ATGP1443-250, ATGP1443-500, ATGP1443-1

Wishart MJ. et al. (1995) J. Biol. Chem. 270:26782-26785.; ; Wishart MJ. et al. (2002) Proc Natl Acad Sci u S A. 99:2112-2117.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH8.5) containing 40% glycerol, 1mM DTT, 0.1M NaCl

Enzymes & Proteases

NP_660294

Q8WUJ0

Human

MGSSHHHHHH SSGLVPRGSH MGSHMEDVKL EFPSLPQCKE DAEEWTYPMR REMQEILPGL FLGPYSSAMK SKLPVLQKHG ITHIICIRQN IEANFIKPNF QQLFRYLVLD IADNPVENII RFFPMTKEFI DGSLQMGGKV LVHGNAGISR SAAFVIAYIM ETFGMKYRDA FAYVQERRFC INPNAGFVHQ LQEYEAIYLA KLTIQMMSPL QIERSLSVHS GTTGSLKRTH EEEDDFGTMQ VATAQNG