Recombinant humaan STYX-eiwit

STYX, also known as serine/threonine/tyrosine-interacting protein, belongs to the protein-tyrosine phosphatase family. It contains a Gly residue instead of a conserved Cys residue in the dsPTPase catalytic loop which renders it catalytically inactive as a phosphatase. The binding pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. It seems to play a role in spermiogenesis. Recombinant human STYX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Serine/threonine/tyrosine-interacting protein, FLJ42934

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

25.0 kDa (221aa) confirmed by MALDI-TOF

STYX, Serine/threonine/tyrosine-interacting protein, FLJ42934, ATGP1452-10 µg, ATGP1452-20 µg, ATGP1452-50 µg, ATGP1452-100 µg, ATGP1452-250 µg, ATGP1452-500 µg, ATGP1452-1 mg, ATGP1452-10, ATGP1452-20, ATGP1452-50, ATGP1452-100, ATGP1452-250, ATGP1452-500, ATGP1452-1

Wishart MJ. et al. (1995) J. Biol. Chem. 270:26782-26785.; ; Wishart MJ. et al. (2002) Proc Natl Acad Sci u S A. 99:2112-2117.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Enzymes & Proteases

NP_660294

Q8WUJ0

Human

MGSSHHHHHH SSGLVPRGSH SHMRREMQEI LPGLFLGPYS SAMKSKLPVL QKHGITHIIC IRQNIEANFI KPNFQQLFRY LVLDIADNPV ENIIRFFPMT KEFIDGSLQM GGKVLVHGNA GISRSAAFVI AYIMETFGMK YRDAFAYVQE RRFCINPNAG FVHQLQEYEA IYLAKLTIQM MSPLQIERSL SVHSGTTGSL KRTHEEEDDF GTMQVATAQN G