Recombinant humaan SUB1-eiwit

SuB1, also known as PC4, is a general coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SuB1 interacts with the activation domain of transcription factor IIA (TFIIA) and TATA-binding protein (TBP) -associated factors (TAFs) to directly bind to double stranded DNA. This protein induces both activation and repression of RNAPII basal transcription, depending on the presence or absence of these transcription factors and holoenzyme components. Recombinant human SuB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

SUB1 regulator of transcriptio, SUB1 homolog transcriptional regulator, Activated RNA polymerase II transcriptional coactivator p15, Positive cofactor 4, PC4, SUB1 homolog, p14, PC4, RPO2TC1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

16.5 kDa (147aa) confirmed by MALDI-TOF

SuB1, Activated RNA polymerase II transcriptional coactivator p15, p14, P15, PC4, Activated RNA polymerase II transcriptional coactivator p15 Activated RNA polymerase II transcriptional coactivator p15, Interferon related developmental regulator 1, MGC102747, PC4 LSB, Positive cofactor 4, RPO2TC1, SuB1, SuB1 homolog., ATGP0573-10 µg, ATGP0573-20 µg, ATGP0573-50 µg, ATGP0573-100 µg, ATGP0573-250 µg, ATGP0573-500 µg, ATGP0573-1 mg, ATGP0573-10, ATGP0573-20, ATGP0573-50, ATGP0573-100, ATGP0573-250, ATGP0573-500, ATGP0573-1

Tavenet A., et al. (2009) Proc Natl Acad Sci u S A. 106 (34) :14265-70. ; ; Scott KL., et al. (2009) Nature. 459 (7250) :1085-90.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

Epigenomics (Transcription & Translation)

NP_006704.3

P53999

Human

MPKSKELVSS SSSGSDSDSE VDKKLKRKKQ VAPEKPVKKQ KTGETSRALS SSKQSSSSRD DNMFQIGKMR YVSVRDFKGK VLIDIREYWM DPEGEMKPGR KGISLNPEQW SQLKEQISDI DDAVRKL