Recombinant humaan Sulfatase Modifying Factor 1/SUMF1-eiwit

SuMF1 (Sulfatase-modifying factor 1) belongs to the SuMF family. SuMF1 is an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine. Mutations in this gene cause multiple sulfatase deficiency, a lysosomal storage disorder. Recombinant human SuMF1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

SUMF1, AAPA3037, FGE, UNQ3037, Formylglycine-generating enzyme, C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.1 kDa (304aa)

UNQ3037, SUMF1, SUMF 1, Sulfatase-modifying factor1, Sulfatase-modifying factor 1, Sulfatase modifying factor1, Sulfatase modifying factor 1, Formylglycine-generating enzyme, Formylglycine generating enzyme, FGE, C-alpha-formylglycine-generating enzyme1, C-alpha-formylglycine-generating enzyme 1, C alpha formylglycine generating enzyme1, C alpha formylglycine generating enzyme 1, AAPA3037, ATGP1301-10 µg, ATGP1301-20 µg, ATGP1301-50 µg, ATGP1301-100 µg, ATGP1301-250 µg, ATGP1301-500 µg, ATGP1301-1 mg, ATGP1301-010, ATGP1301-020, ATGP1301-050, ATGP1301-100, ATGP1301-250, ATGP1301-500, ATGP1301-01M

Fraldi A., et al. (2007) Biochem J. 403 (2) :305-12.; ; Cosma MP., et al. (2003) Cell 113 (4) : 445-56.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 20% glycerol, 2mM DTT

Cytoskeleton

NP_877437

Q8NBK3

Human

MVPIPAGVFT MGTDDPQIKQ DGEAPARRVT IDAFYMDAYE VSNTEFEKFV NSTGYLTEAE KFGDSFVFEG MLSEQVKTNI QQAVAAAPWW LPVKGANWRH PEGPDSTILH RPDHPVLHVS WNDAVAYCTW AGKRLPTEAE WEYSCRGGLH NRLFPWGNKL QPKGQHYANI WQGEFPVTNT GEDGFQGTAP VDAFPPNGYG LYNIVGNAWE WTSDWWTVHH SVEETLNPKG PPSGKDRVKK GGSYMCHRSY CYRYRCAARS QNTPDSSASN LGFRCAADRL PTMD