Recombinant humaan Sulfatase Modifying Factor 1/SUMF1-eiwit

SUMF1, also known as sulfatase-modifying factor 1 isoform 1, is a Ca2+-binging member of the sulfatase-modifying factor family. This protein as soluble ER lumenal glycoprotein converts inactive sulfatases into an active form by transforming a catalytic site cysteine into a formylglycine residue. In the ER, it can exist as either a monomer, or a disulfide-linked homodimer or a heterodimer with SUMF2. The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in inactive FGE, and subsequently multiple sulfatase deficiency, a lysosomal storage disorder. Recombinant Human SUMF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

SUMF1, AAPA3037, FGE, UNQ3037, Formylglycine-generating enzyme, C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

38.1 kDa (347aa)

UNQ3037, SUMF1, SUMF 1, Sulfatase-modifying factor1, Sulfatase-modifying factor 1, Sulfatase modifying factor1, Sulfatase modifying factor 1, Formylglycine-generating enzyme, Formylglycine generating enzyme, FGE, C-alpha-formylglycine-generating enzyme1, C-alpha-formylglycine-generating enzyme 1, C alpha formylglycine generating enzyme1, C alpha formylglycine generating enzyme 1, AAPA3037, ATGP3893-10 µg, ATGP3893-20 µg, ATGP3893-50 µg, ATGP3893-100 µg, ATGP3893-250 µg, ATGP3893-500 µg, ATGP3893-1 mg, ATGP3893-010, ATGP3893-020, ATGP3893-050, ATGP3893-100, ATGP3893-250, ATGP3893-500, ATGP3893-01M

Preusser-Kunze A., et al, (2005) J. Biol. Chem. 280:14900-14910.; ; Zito E., et al, (2005) EMBO Rep. 6:655-660.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cytoskeleton

NP_877437.2

Q8NBK3

Human

Ser34