Recombinant humaan Sulfotransferase 1A2/SULT1A2-eiwit

Sulfotransferase 1A2, also known as SuLT1A2, is a member of sulfotransferase family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. It mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. Recombinant human SuLT1A2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Sulfotransferase family 1A member 2, ST1A2, Aryl sulfotransferase 2, Phenol sulfotransferase 2, Phenol-sulfating phenol sulfotransferase 2, P-PST 2, STP2, sulfotransferase family cytosolic 1A phenol-preferring member 2, HAST4

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

36.4 kDa (315aa) confirmed by MALDI-TOF

SULT1A2, Sulfotransferase 1A2, HAST4, P-PST, ST1A2, STP2, TSPST2, ATGP1135-10 µg, ATGP1135-20 µg, ATGP1135-50 µg, ATGP1135-100 µg, ATGP1135-250 µg, ATGP1135-500 µg, ATGP1135-1 mg, ATGP1135-10, ATGP1135-20, ATGP1135-50, ATGP1135-100, ATGP1135-250, ATGP1135-500, ATGP1135-1

Weinshilboum R M., et al. (1997) FASEB J. 11 (1) :3-14.; ; Carlini E J., et al. (2001) Pharmacogenetics. 11 (1) :57-68.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Metabolism

NP_001045

P50226

Human

MELIQDISRP PLEYVKGVPL IKYFAEALGP LQSFQARPDD LLISTYPKSG TTWVSQILDM IYQGGDLEKC HRAPIFMRVP FLEFKVPGIP SGMETLKNTP APRLLKTHLP LALLPQTLLD QKVKVVYVAR NAKDVAVSYY HFYHMAKVYP HPGTWESFLE KFMAGEVSYG SWYQHVQEWW ELSRTHPVLY LFYEDMKENP KREIQKILEF VGRSLPEETV DLMVEHTSFK EMKKNPMTNY TTVRREFMDH SISPFMRKGM AGDWKTTFTV AQNERFDADY AEKMAGCSLS FRSEL