Recombinant humaan Sulfotransferase 1B1/SULT1B1-eiwit

Sulfotransferase family, cytosolic, 1B, member 1, also known as SuLT1B1, is an enzyme that in humans is encoded by the SuLT1B1 gene. SuLT1B1 contains a binding site for the sulfate donor, 3-prime-phosphoadenosine 5-prime-phosphosulfate, and a cysteine residue conserved in the ST1 gene family of sulfotransferases. Sulfotransferases such as SuLT1B1 catalyze the biotransformation of a large number of endogenous compounds such as neurotransmitters, steroids, bile acids, and thyroid hormones, as well as drugs and xenobiotics. Recombinant human SuLT1B1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sulfotransferase family cytosolic 1B member 1, Sulfotransferase family, cytosolic, 1B, member 1, ST1B1, ST1B2, SuLT1B2, Thyroid hormone sulfotransferase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

37.4 kDa (320aa) confirmed by MALDI-TOF

SULT1B1, Sulfotransferase family cytosolic 1B member 1, Sulfotransferase family, cytosolic, 1B, member 1, ST1B1, ST1B2, SuLT1B2, ATGP1537-10 µg, ATGP1537-20 µg, ATGP1537-50 µg, ATGP1537-100 µg, ATGP1537-250 µg, ATGP1537-500 µg, ATGP1537-1 mg, ATGP1537-10, ATGP1537-20, ATGP1537-50, ATGP1537-100, ATGP1537-250, ATGP1537-500, ATGP1537-1

Meinl W., et al. (2001) Biochem Biophys Res Commun. 288 (4) :855-62.; ; Glatt H., et al. (2000) Toxicol Lett. 113: 341-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Metabolism

NP_055280

O43704

Human

MGSSHHHHHH SSGLVPRGSH MGSHMLSPKD ILRKDLKLVH GYPMTCAFAS NWEKIEQFHS RPDDIVIATY PKSGTTWVSE IIDMILNDGD IEKCKRGFIT EKVPMLEMTL PGLRTSGIEQ LEKNPSPRIV KTHLPTDLLP KSFWENNCKM IYLARNAKDV SVSYYHFDLM NNLQPFPGTW EEYLEKFLTG KVAYGSWFTH VKNWWKKKEE HPILFLYYED MKENPKEEIK KIIRFLEKNL NDEILDRIIH HTSFEVMKDN PLVNYTHLPT TVMDHSKSPF MRKGTAGDWK NYFTVAQNEK FDAIYETEMS KTALQFRTEI