Recombinant humaan Sulfotransferase 1C4/SULT1C4-eiwit

Sulfotransferase 1C4, also known as SuLT1C4, belongs to the SuLT subfamily, responsible for transferring a sulfo moiety from PAPS to phenol-containing compounds. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SuLT1C4 catalyzes the sulfonation of p-nitrophenol and N-hydroxy-2-acetylaminofluorene, but not dopamine. Recombinant human SuLT1C4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sulfotransferase family 1C member 4, Sulfotransferase 1C4, ST1C4, Sulfotransferase 1C2, SULT1C2, Sulfotransferase family cytosolic 1C member 2, Sulfotransferase family cytosolic 1C member 4, SULT1C

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

37.6 kDa (322aa) confirmed by MALDI-TOF

SULT1C4, Sulfotransferase 1C4, SuLT1C, SuLT1C2, ATGP2110-10 µg, ATGP2110-20 µg, ATGP2110-50 µg, ATGP2110-100 µg, ATGP2110-250 µg, ATGP2110-500 µg, ATGP2110-1 mg, ATGP2110-10, ATGP2110-20, ATGP2110-50, ATGP2110-100, ATGP2110-250, ATGP2110-500, ATGP2110-1

Sakakibara Y., et al. (1998) J Biol Chem. 273 (51) :33929-35

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Metabolism

NP_006579

O75897

Human

MALHDMEDFT FDGTKRLSVN YVKGILQPTD TCDIWDKIWN FQAKPDDLLI STYPKAGTTW TQEIVELIQN EGDVEKSKRA PTHQRFPFLE MKIPSLGSGL EQAHAMPSPR ILKTHLPFHL LPPSLLEKNC KIIYVARNPK DNMVSYYHFQ RMNKALPAPG TWEEYFETFL AGKVCWGSWH EHVKGWWEAK DKHRILYLFY EDMKKNPKHE IQKLAEFIGK KLDDKVLDKI VHYTSFDVMK QNPMANYSSI PAEIMDHSIS PFMRKGAVGD WKKHFTVAQN ERFDEDYKKK MTDTRLTFHF QF