Recombinant humaan Sulfotransferase 2B1/SULT2B1-eiwit

Sulfotransferase family cytosolic 2B member 1 isoform b, also known as SuLT2B1, is a member of Sulfotransferase family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SuLT2B1b is localized in the cytosol and nuclei of human cells. SuLT2B1b is selective for the sulfation of 3beta-hydroxysteroids such as dehydroepiandrosterone and pregnenolone, and may also have a role in cholesterol sulfation in human skin. Recombinant human SuLT2B1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sulfotransferase family 2B member 1, Sulfotransferase 2B1, Alcohol sulfotransferase, Hydroxysteroid sulfotransferase 2, Sulfotransferase family cytosolic 2B member 1, ST2B1, HSST2

E.coli

Human

Non-Tagged

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

41.3 kDa (365aa) confirmed by MALDI-TOF

Cytosolic Sulfotransferase2B1, SULT2B1, Sulfotransferase family cytosolic 2B member 1 isoform b, HSST2, ST2B1, Sulfotransferase family cytosolic 2B member 1 isoform b Alcohol sulfotransferase, EC 2.8.2.2, Hydroxysteroid sulfotransferase 2, Sulfotransferase 2B1, Sulfotransferase family cytosolic 2B member 1, SuLT2B1a, SuLT2B1b., ATGP0510-10 µg, ATGP0510-20 µg, ATGP0510-50 µg, ATGP0510-100 µg, ATGP0510-250 µg, ATGP0510-500 µg, ATGP0510-1 mg, ATGP0510-10, ATGP0510-20, ATGP0510-50, ATGP0510-100, ATGP0510-250, ATGP0510-500, ATGP0510-1

Dumas NA., et al. (2008) J Steroid Biochem Mol Biol. 111:171-7. ; ; He D., et al. (2005) Biolchim Biophys Acta. 1724:11-26.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Metabolism

NP_814444

O00204

Human

MDGPAEPQIP GLWDTYEDDI SEISQKLPGE YFRYKGVPFP VGLYSLESIS LAENTQDVRD DDIFIITYPK SGTTWMIEII CLILKEGDPS WIRSVPIWER APWCETIVGA FSLPDQYSPR LMSSHLPIQI FTKAFFSSKA KVIYMGRNPR DVVVSLYHYS KIAGQLKDPG TPDQFLRDFL KGEVQFGSWF DHIKGWLRMK GKDNFLFITY EELQQDLQGS VERICGFLGR PLGKEALGSV VAHSTFSAMK ANTMSNYTLL PPSLLDHRRG AFLRKGVCGD WKNHFTVAQS EAFDRAYRKQ MRGMPTFPWD EDPEEDGSPD PEPSPEPEPK PSLEPNTSLE REPRPNSSPS PSPGQASETP HPRPS