Recombinant humaan SUMO E1/SAE1-eiwit

SAE1, also known as AOS1, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the uBL1 conjugation pathway. Proteins conjugated to ub are marked for progressive degradation by the 26S Proteasome. SAE1, which is a dimeric enzyme, functions as a uBLI E1 ligase mediating the ATP-dependent activation of uBL1. This protein can bind with uBLE1A and uBLE1B to form a heterodimer which can bind uBL1. Recombinant human SAE1 protein, fused to His-T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

SUMO1 activating enzyme subunit 1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, activator Of sumo 1, AOS1, SUA1, UBLE1A

E.coli

Human

His-T7-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

42.2 kDa (378aa) confirmed by MALDI-TOF

SAE1, SuMO1 activating enzyme subunit 1, AOS1, HSPC140, SuA1, uBLE1A, SuMO1 activating enzyme subunit 1, ubiquitin-like 1-activating enzyme E1A, AOS 1, HSPC 140, SuA 1, uBLE 1A, uBL E1A, SAE 1, ubiquitin like protein SuMO 1 activating enzyme, ATGP0610-10 µg, ATGP0610-20 µg, ATGP0610-50 µg, ATGP0610-100 µg, ATGP0610-250 µg, ATGP0610-500 µg, ATGP0610-1 mg, ATGP0610-10, ATGP0610-20, ATGP0610-50, ATGP0610-100, ATGP0610-250, ATGP0610-500, ATGP0610-1

Boggio R., et al. (2007) J Biol Chem. 282 (21) :15376-82.; ; Jones MC., et al. (2006) Proc Natl Acad Sci u S A. 103 (44) :16272-7.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Ubiquitination

NP_005491.1

Q9UBE0

Human

MVEKEEAG GGISEEEAAQ YDRQIRLWGL EAQKRLRASR VLLVGLKGLG AEIAKNLILA GVKGLTMLDH EQVTPEDPGA QFLIRTGSVG RNRAEASLER AQNLNPMVDV KVDTEDIEKK PESFFTQFDA VCLTCCSRDV IVKVDQICHK NSIKFFTGDV FGYHGYTFAN LGEHEFVEEK TKVAKVSQGV EDGPDTKRAK LDSSETTMVK KKVVFCPVKE ALEVDWSSEK AKAALKRTTS DYFLLQVLLK FRTDKGRDPS SDTYEEDSEL LLQIRNDVLD SLGISPDLLP EDFVRYCFSE MAPVCAVVGG ILAQEIVKAL SQRDPPHNNF FFFDGMKGNG IVECLGPK