Recombinant humaan SUMO1-eiwit

SuMO1, also known as small ubiquitin-related modifier 1, is a member of the SuMO protein family and functions in a manner similar to ubiquitin. However, unlike ubiquitin which targets proteins for degradation, SuMO1 protein participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Recombinant human SuMO1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Small ubiquitin-related modifier 1, DAP-1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, SuMO-1, uBL1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

12.6 kDa (109aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

SUMO1, small ubiquitin-related modifier 1, DAP-1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, SuMO-1, uBL1, ATGP1293-10 µg, ATGP1293-20 µg, ATGP1293-50 µg, ATGP1293-100 µg, ATGP1293-250 µg, ATGP1293-500 µg, ATGP1293-1 mg, ATGP1293-10, ATGP1293-20, ATGP1293-50, ATGP1293-100, ATGP1293-250, ATGP1293-500, ATGP1293-1

Evdokimov E., et al. (2008) J Cell Sci. 121:4106-13.; ; Hecker CM., et al. (2006) J Biol Chem. 281 (23) :16117-27.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl

Ubiquitination

NP_003343

P63165

Human

MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGGHST VLEHHHHHH