Recombinant humaan SUOX-eiwit

SuOX also known as sulfite oxidase. SuOX is a homodimeric protein localized to the intermembrane space of mitochondria. Each subunit contains a heme domain and a molybdopterin-binding domain. This enzyme catalyzes the oxidation of sulfite to sulfate, the final reaction in the oxidative degradation of the sulfur amino acids cysteine and methionine. SuOX deficiency results in neurological abnormalities which are often fatal at an early age. Recombinant human SuOX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sulfite oxidase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

53.9 kDa (489aa)

SuOX, Sulfite oxidase, Sulfite oxidase, EC 1.8.3.1, ATGP3022-10 µg, ATGP3022-20 µg, ATGP3022-50 µg, ATGP3022-100 µg, ATGP3022-250 µg, ATGP3022-500 µg, ATGP3022-1 mg, ATGP3022-10, ATGP3022-20, ATGP3022-50, ATGP3022-100, ATGP3022-250, ATGP3022-500, ATGP3022-1

Rudolph M.J., et al. (2003) Acta Crystallogr. D 59:1183-1191.; ; Kisker C., et al. (1997) Cell 91:973-983.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Metabolism

NP_000447.2

P51687

Human

ESTHIYT KEEVSSHTSP ETGIWVTLGS EVFDVTEFVD LHPGGPSKLM LAAGGPLEPF WALYAVHNQS HVRELLAQYK IGELNPEDKV APTVETSDPY ADDPVRHPAL KVNSQRPFNA EPPPELLTEN YITPNPIFFT RNHLPVPNLD PDTYRLHVVG APGGQSLSLS LDDLHNFPRY EITVTLQCAG NRRSEMTQVK EVKGLEWRTG AISTARWAGA RLCDVLAQAG HQLCETEAHV CFEGLDSDPT GTAYGASIPL ARAMDPEAEV LLAYEMNGQP LPRDHGFPVR VVVPGVVGAR HVKWLGRVSV QPEESYSHWQ RRDYKGFSPS VDWETVDFDS APSIQELPVQ SAITEPRDGE TVESGEVTIK GYAWSGGGRA VIRVDVSLDG GLTWQVAKLD GEEQRPRKAW AWRLWQLKAP VPAGQKELNI VCKAVDDGYN VQPDTVAPIW NLRGVLSNAW HRVHVYVSP