Recombinant humaan SYCE3-eiwit

SYCE3 (synaptonemal complex central element protein 3) is major component of the transverse central element of synaptonemal complexes (SCS) . The synaptonemal complex is a protein structure that forms between homologous chromosomes (two pairs of sister chromatids) during meiosis and that is thought to mediate chromosome pairing, synapsis, and recombination (crossing-over) . This protein required for chromosome loading of the central element-specific SCS proteins, and for initiating synapsis between homologous chromosomes. Recombinant human SYCE3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Synaptonemal complex central element protein 3, testis highly expressed protein 2, C22orf41, chromosome 22 open reading frame 41, THEG-2, THEG2, SYCE-3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

12.8 kDa (108aa) confirmed by MALDI-TOF

SYCE3, synaptonemal complex central element protein 3, testis highly expressed protein 2, C22orf41, chromosome 22 open reading frame 41, THEG-2, THEG2, SYCE-3, ATGP1672-10 µg, ATGP1672-20 µg, ATGP1672-50 µg, ATGP1672-100 µg, ATGP1672-250 µg, ATGP1672-500 µg, ATGP1672-1 mg, ATGP1672-10, ATGP1672-20, ATGP1672-50, ATGP1672-100, ATGP1672-250, ATGP1672-500, ATGP1672-1

Schramm S, et al. (2011) PLoS Genet, May; ;7 (5) :e1002088

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT

Cell Cycle

NP_001116697

A1L190

Human

MGSSHHHHHH SSGLVPRGSH MDDADPEERN YDNMLKMLSD LNKDLEKLLE EMEKISVQAT WMAYDMVVMR TNPTLAESMR RLEDAFVNCK EEMEKNWQEL LHETKQRL