Recombinant humaan SYF2-eiwit

SYF2, also known as pre-mRNA-splicing factor SYF2, may be involved in pre-mRNA splicing. This protein interacts with cyclin D-type binding-protein 1, which is thought to be a cell cycle regulator at the G1/S transition. It is abundantly expressed in the heart, skeletal muscle and kidney and is expressed at lower levels in other tissues. Recombinant human SYF2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Pre-mRNA-splicing factor SYF2, CBPIN, GCIPIP, p29

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

20.5 kDa (173aa) confirmed by MALDI-TOF

SYF2, Pre-mRNA-splicing factor SYF2, CBPIN, GCIPIP, p29, ATGP1269-10 µg, ATGP1269-20 µg, ATGP1269-50 µg, ATGP1269-100 µg, ATGP1269-250 µg, ATGP1269-500 µg, ATGP1269-1 mg, ATGP1269-10, ATGP1269-20, ATGP1269-50, ATGP1269-100, ATGP1269-250, ATGP1269-500, ATGP1269-1

Chang M.-S., et al. (2000) Biochem. Biophys. Res. Commun. 279:732-737; ; Jurica M.S., et al. (2002) RNA. 8:426-439

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl, 5mM DTT, 2mM EDTA

Cell Cycle

NP_056299

O95926

Human

DYEKVKL LEISAEDAER WERKKKRKNP DLGFSDYAAA QLRQYHRLTK QIKPDMETYE RLREKHGEEF FPTSNSLLHG THVPSTEEID RMVIDLEKQI EKRDKYSRRR PYNDDADIDY INERNAKFNK KAERFYGKYT AEIKQNLERG TAV