Recombinant humaan Synaptotagmin-1 eiwit

Synaptotagmin-1 (SYT1) belongs to the synaptotagmin family contains 2 C2 domains. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis. SYT1 is the master switch responsible for allowing the human brain to release neurotransmitters. Recombinant human SYT1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Synaptotagmin 1, P65, SVP65, SYT

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

29.5 kDa (256aa) confirmed by MALDI-TOF

Synaptotagmin1, synaptotagmin 1, P65, SVP65, SYT, ATGP1091-10 µg, ATGP1091-20 µg, ATGP1091-50 µg, ATGP1091-100 µg, ATGP1091-250 µg, ATGP1091-500 µg, ATGP1091-1 mg, ATGP1091-10, ATGP1091-20, ATGP1091-50, ATGP1091-100, ATGP1091-250, ATGP1091-500, ATGP1091-1

Han C., et al. (2009) J. Immunol. 182:2986-2996; ; Xi, D., et al. (1999) Neuroscience 88: 425-435.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100 mM NaCl

Neuroscience

NP_005630

P21579

Human

MEPKEEEKLG KLQYSLDYDF QNNQLLVGII QAAELPALDM GGTSDPYVKV FLLPDKKKKF ETKVHRKTLN PVFNEQFTFK VPYSELGGKT LVMAVYDFDR FSKHDIIGEF KVPMNTVDFG HVTEEWRDLQ SAEKEEQEKL GDICFSLRYV PTAGKLTVVI LEAKNLKKMD VGGLSDPYVK IHLMQNGKRL KKKKTTIKKN TLNPYYNESF SFEVPFEQIQ KVQVVVTVLD YDKIGKNDAI GKVFVGYNLE HHHHHH