Recombinant humaan Syndecaan-1/SDC1-eiwit

Syndecan-1 precursor, also known as SDC1, is a transmembrane heparin sulphate proteoglycan which is made up of one core protein and five glycosaminoglycan. SDC1 is expected to play a role in cell adhesion. It is expressed on the surface of pre-B cells and plasma cells but is absent from mature B cells. It is a selective marker for B cell lymphoblastic leukemia and lymphoplasmocytoid leukemia. Recombinant human SDC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Syndecan-1 precursor, CD138, SDC, SYND1, Syndecan, Syndecan proteoglycan 1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

27 kDa (262aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Syndecan1, CD138, Syndecan-1 precursor, CD138, SDC, SYND1, syndecan, ATGP1467-10 µg, ATGP1467-20 µg, ATGP1467-50 µg, ATGP1467-100 µg, ATGP1467-250 µg, ATGP1467-500 µg, ATGP1467-1 mg, ATGP1467-10, ATGP1467-20, ATGP1467-50, ATGP1467-100, ATGP1467-250, ATGP1467-500, ATGP1467-1

O'Connell FP., et al. (2004) Am J Clin Pathol. 121 (2) :254-63-21531.; ; Rawstron AC., et al. (2006) Curr Protoc Cytom. 6: unit6.23.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Metabolism

NP_002988.2

P18827

Human

QPALP QIVATNLPPE DQDGSGDDSD NFSGSGAGAL QDITLSQQTP STWKDTQLLT AIPTSPEPTG LEATAASTST LPAGEGPKEG EAVVLPEVEP GLTAREQEAT PRPRETTQLP TTHQASTTTA TTAQEPATSH PHRDMQPGHH ETSTPAGPSQ ADLHTPHTED GGPSATERAA EDGASSQLPA AEGSGEQDFT FETSGENTAV VAVEPDRRNQ SPVDQGATGA SQGLLDRKEV LG