Recombinant humaan SYT13-eiwit

SYT13 is a member of the large synaptotagmin protein family. Family members have an extracellular N-terminal transmembrane domain and a cytoplasmic C terminus with two tandem C2 domains (C2A and C2B) . Synaptotogmin family members can form homo- and heteromeric complexes with each other. They also have different biochemical properties and developmental profiles, and patterns of tissue distribution. Synaptotagmins function as membrane traffickers in multicellular organisms. Two alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. Recombinant human SYT13 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Synaptotagmin-13 isoform 1, Synaptotagmin-13 isoform 1, Synaptotagmin XIII

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

46.5 kDa (420aa) confirmed by MALDI-TOF

SYT13, Synaptotagmin-13 isoform 1, Synaptotagmin-13 isoform 1, Synaptotagmin XIII, ATGP2203-10 µg, ATGP2203-20 µg, ATGP2203-50 µg, ATGP2203-100 µg, ATGP2203-250 µg, ATGP2203-500 µg, ATGP2203-1 mg, ATGP2203-10, ATGP2203-20, ATGP2203-50, ATGP2203-100, ATGP2203-250, ATGP2203-500, ATGP2203-1

Fukuda M, et al. (2001) . Biochem J. 354 (Pt 2) :249-57.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT, 1mM PMSF, 1mM EDTA

Signal Transduction

NP_065877

Q7L8C5

Human

MGSSHHHHHH SSGLVPRGSH MGSCRHMHPK KGLLPRDQDP DLEKAKPSLL GSAQQFNVKK STEPVQPRAL LKFPDIYGPR PAVTAPEVIN YADYSLRSTE EPTAPASPQP PNDSRLKRQV TEELFILPQN GVVEDVCVME TWNPEKAASW NQAPKLHYCL DYDCQKAELF VTRLEAVTSN HDGGCDCYVQ GSVANRTGSV EAQTALKKRQ LHTTWEEGLV LPLAEEELPT ATLTLTLRTC DRFSRHSVAG ELRLGLDGTS VPLGAAQWGE LKTSAKEPSA GAGEVLLSIS YLPAANRLLV VLIKAKNLHS NQSKELLGKD VSVKVTLKHQ ARKLKKKQTK RAKHKINPVW NEMIMFELPD DLLQASSVEL EVLGQDDSGQ SCALGHCSLG LHTSGSERSH WEEMLKNPRR QIAMWHQLHL