Recombinant humaan SYT4-eiwit

Synaptotagmin-4, also known as SYT4, belongs to the synaptotagmin family proteins. Synaptotagmins are a large gene family of synaptic vesicle type III integral membrane proteins that function as regulators of both exocytosis and endocytosis and are involved in neurotransmitter secretion from small secretory vesicles. SYT4 is expressed in neuronal tissues, and has the highest mRNA levels in the hippocampus. The proximity of the SYT4 gene to markers of several psychiatric disorders suggest an involvement of SYT4 in human diseasee. Recombinant human SYT4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Synaptotagmin-4, HsT1192, KIAA1342

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

46.1 kDa (409aa) confirmed by MALDI-TOF

SYT4, Synaptotagmin-4, HsT1192, KIAA1342, ATGP1982-10 µg, ATGP1982-20 µg, ATGP1982-50 µg, ATGP1982-100 µg, ATGP1982-250 µg, ATGP1982-500 µg, ATGP1982-1 mg, ATGP1982-10, ATGP1982-20, ATGP1982-50, ATGP1982-100, ATGP1982-250, ATGP1982-500, ATGP1982-1

Ferguson G D., et al. (2000) J Biol Chem. 275:36920-36926.; ; Ibata K., et al. (2002) Neurosci Res. 43: 401-406.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_065834

Q9H2B2

Human

MGSSHHHHHH SSGLVPRGSH MCQRKSSKSN KTPPYKFVHV LKGVDIYPEN LNSKKKFGAD DKNEVKNKPA VPKNSLHLDL EKRDLNGNFP KTNLKPGSPS DLENATPKLF LEGEKESVSP ESLKSSTSLT SEEKQEKLGT LFFSLEYNFE RKAFVVNIKE ARGLPAMDEQ SMTSDPYIKM TILPEKKHKV KTRVLRKTLD PAFDETFTFY GIPYTQIQEL ALHFTILSFD RFSRDDIIGE VLIPLSGIEL SEGKMLMNRE IIKRNVRKSS GRGELLISLC YQSTTNTLTV VVLKARHLPK SDVSGLSDPY VKVNLYHAKK RISKKKTHVK KCTPNAVFNE LFVFDIPCEG LEDISVEFLV LDSERGSRNE VIGQLVLGAA AEGTGGEHWK EICDYPRRQI AKWHVLCDG