Recombinant humaan t-plasminogeenactivator/PLAT-eiwit

PLAT, as known as tissue-type plasminogen activator isoform 1, is a secreted serine protease synthesized by endothelial cells. This protein is secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Also, it plays a role clinical medicine to treat stroke in cell migration and tissue remodeling. It is implicated in normal neural function and is suggested as a regulatory molecule in neurodegenerative diseases. Recombinant human PLAT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Tissue-type plasminogen activator isoform 1, PLAT, T-PA, TPA

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

61.3 kDa (545aa)

T-Plasminogen Activator, Tpa, Tissue-type plasminogen activator isoform 1, PLAT, T-PA, TPA, ATGP3486-10 µg, ATGP3486-20 µg, ATGP3486-50 µg, ATGP3486-100 µg, ATGP3486-250 µg, ATGP3486-500 µg, ATGP3486-1 mg, ATGP3486-10, ATGP3486-20, ATGP3486-50, ATGP3486-100, ATGP3486-250, ATGP3486-500, ATGP3486-1

Chevilley A., et al, (2015) Front Cell Neurosci. 9:415.; ; Lansley SM., et al, (2015) Am. J. Respir. Cell Mol. Biol. 53:105-112.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 50 mM MES buffer (pH 5.5) containing 40% glycerol, 5mM CaCl2, 1mM DTT, 0.5M NaCl

Cardiovascular

NP_000921

P00750

Human

Gln24