Recombinant humaan t-plasminogeenactivator/PLAT-eiwit

PLAT, also known as plasminogen activator, tissue type, is serine protease secreted by many cell types that participate in biological processes, such as tissue restructuring, cell migration, and tumor metastasis. It used to dissolve coronary fibrin clots that are the proximal causes of acute myocardial infarction. It plays a crucial role in regulating blood fibrinolysis, in maintaining the homeostasis of extracellular matrix and in modulating the post-translational activation of growth factors. Recombinant human PLAT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Plasminogen activator, tissue type, PLAT, T-PA, TPA

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

29.2 kDa (261aa)

T-Plasminogen Activator, tPA, Plasminogen activator, tissue type, PLAT, T-PA, TPA, ATGP3504-10 µg, ATGP3504-20 µg, ATGP3504-50 µg, ATGP3504-100 µg, ATGP3504-250 µg, ATGP3504-500 µg, ATGP3504-1 mg, ATGP3504-10, ATGP3504-20, ATGP3504-50, ATGP3504-100, ATGP3504-250, ATGP3504-500, ATGP3504-1

Orth K., et al. (1992) Proc Natl Acad Sci U S A. 89:7422-7426.; ; Hu K., et al. (2008) Front Biosci. 13:5174-5186.

Hirayama S, et al. Triosephosphate isomerase of Streptococcus pneumoniae is released extracellularly by autolysis and binds to host plasminogen to promote its activation. (FEBS Open Bio. 2022) {https://pubmed.ncbi.nlm.nih.gov/35298875/}; ; Hirayama S, et al. C-Terminal Lysine Residue of Pneumococcal Triosephosphate Isomerase Contributes to Its Binding to Host Plasminogen. (Microorganisms. 2023) {https://pubmed.ncbi.nlm.nih.gov/37317172/}; ; Yasui Y, et al. The Pneumococcal Protein SufC Binds to Host Plasminogen and Promotes Its Conversion into Plasmin. (Microorganisms. 2023) {https://pubmed.ncbi.nlm.nih.gov/38138113/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 50 mM MES buffer (pH 5.0) containing 5mM CaCl2, 1mM DTT, 0.5M NaCl, 30% glycerol

Cardiovascular

NP_000921.1

P00750

Human

Ala

IKGGLFA DIASHPWQAA IFAKHRRSPG ERFLCGGILI SSCWILSAAH CFQERFPPHH LTVILGRTYR VVPGEEEQKF EVEKYIVHKE FDDDTYDNDI ALLQLKSDSS RCAQESSVVR TVCLPPADLQ LPDWTECELS GYGKHEALSP FYSERLKEAH VRLYPSSRCT SQHLLNRTVT DNMLCAGDTR SGGPQANLHD ACQGDSGGPL VCLNDGRMTL VGIISWGLGC GQKDVPGVYT KVTNYLDWIR DNMRP