Recombinant humaan t-plasminogeenactivator/PLAT-eiwit

PLAT, also known as tissue-type plasminogen activator isoform 1, is a protein involved in the breakdown of blood clots. This protein is secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Also, active PLAT converts plasminogen to plasmin, a fibrinolytic protease, by hydrolyzing an Arg-Val peptide bond in plasminogen. Recombinant human PLAT protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Tissue-type plasminogen activator isoform 1, PLAT, T-PA, TPA

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

32 kDa (284aa)

T-Plasminogen Activator, tPA, Tissue-type plasminogen activator isoform 1, PLAT, T-PA, TPA, ATGP3614-10 µg, ATGP3614-20 µg, ATGP3614-50 µg, ATGP3614-100 µg, ATGP3614-250 µg, ATGP3614-500 µg, ATGP3614-1 mg, ATGP3614-10, ATGP3614-20, ATGP3614-50, ATGP3614-100, ATGP3614-250, ATGP3614-500, ATGP3614-1

Chevilley A., et al, (2015) Front Cell Neurosci. 9:415.; ; Lansley SM., et al, (2015) Am. J. Respir. Cell Mol. Biol. 53:105-112.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 50 mM MES buffer (pH 5.5) containing 10% glycerol, 100 mM NaCl, 5mM CaCl2.

Cardiovascular

NP_000921

P00750

Human

Ala

SYQVICR DEKTQMIYQQ HQSWLRPVLR SNRVEYCWCN SGRAQCHSVP VKSCSEPRCF NGGTCQQALY FSDFVCQCPE GFAGKCCEID TRATCYEDQG ISYRGTWSTA ESGAECTNWN SSALAQKPYS GRRPDAIRLG LGNHNYCRNP DRDSKPWCYV FKAGKYSSEF CSTPACSEGN SDCYFGNGSA YRGTHSLTES GASCLPWNSM ILIGKVYTAQ NPSAQALGLG KHNYCRNPDG DAKPWCHVLK NRRLTWEYCD VPSCSTCGLR QYSQPQFR