Recombinant humaan TACO1-eiwit

TACO1 is a mitochondrial protein that function as a translational activator of mitochondrially-encoded cytochrome c oxidase 1. Mutations in this gene are associated with Leigh syndrome. Recombinant human TACO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Translational activator of cytochrome c oxidase 1, Translational activator of cytochrome c oxidase 1, CCDC44

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

28.8 kDa (260aa) confirmed by MALDI-TOF

TACO1, Translational activator of cytochrome c oxidase 1, Translational activator of cytochrome c oxidase 1, CCDC44, ATGP2400-10 µg, ATGP2400-20 µg, ATGP2400-50 µg, ATGP2400-100 µg, ATGP2400-250 µg, ATGP2400-500 µg, ATGP2400-1 mg, ATGP2400-10, ATGP2400-20, ATGP2400-50, ATGP2400-100, ATGP2400-250, ATGP2400-500, ATGP2400-1

Weraarpachai W., et al. (2009) Nat. Genet. 41:833-837; ; Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_057444

Q9BSH4

Human

MGSSHHHHHH SSGLVPRGSH MGSNKWSKVR HIKGPKDVER SRIFSKLCLN IRLAVKEGGP NPEHNSNLAN ILEVCRSKHM PKSTIETALK MEKSKDTYLL YEGRGPGGSS LLIEALSNSS HKCQADIRHI LNKNGGVMAV GARHSFDKKG VIVVEVEDRE KKAVNLERAL EMAIEAGAED VKETEDEEER NVFKFICDAS SLHQVRKKLD SLGLCSVSCA LEFIPNSKVQ LAEPDLEQAA HLIQALSNHE DVIHVYDNIE