Recombinant humaan TARS1-eiwit

TARS (Threonyl-tRNA synthetase, cytoplasmic) belongs to the class-II aminoacyl-tRNA synthetase family. This protein has its main role in tRNA aminoacylation. The N-terminal domain of the enzyme is responsible for the competition with the ribosome whilst the catalytic and the C-terminal domain are involved in binding the two anticodon arm-like structures in the operator. Recombinant human TARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Threonyl-tRNA synthetase cytoplasmic, threonyl-tRNA synthetase, cytoplasmic, ThrRS

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

85.6 kDa (743aa)

TDP43, TARDBP, threonyl-tRNA synthetase cytoplasmic, threonyl-tRNA synthetase, cytoplasmic, ThrRS, ATGP1417-10 µg, ATGP1417-20 µg, ATGP1417-50 µg, ATGP1417-100 µg, ATGP1417-250 µg, ATGP1417-500 µg, ATGP1417-1 mg, ATGP1417-10, ATGP1417-20, ATGP1417-50, ATGP1417-100, ATGP1417-250, ATGP1417-500, ATGP1417-1

Freist W, et al. (1995) . Biol. Chem. Hoppe-Seyler 376 (4) : 213-24.; ; Pan F, et al. (1982) . Int. J. Pept. Protein Res. 20 (2) : 159-66.

Wang W, et al. The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. (Nat Med. 2016) {https://pubmed.ncbi.nlm.nih.gov/27348499/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150 mM NaCl

Metabolism

NP_689508

P26639

Human

MFEEKASSPS GKMGGEEKPI GAGEEKQKEG GKKKNKEGSG DGGRAELNPW PEYIYTRLEM YNILKAEHDS ILAEKAEKDS KPIKVTLPDG KQVDAESWKT TPYQIACGIS QGLADNTVIA KVNNVVWDLD RPLEEDCTLE LLKFEDEEAQ AVYWHSSAHI MGEAMERVYG GCLCYGPPIE NGFYYDMYLE EGGVSSNDFS SLEALCKKII KEKQAFERLE VKKETLLAMF KYNKFKCRIL NEKVNTPTTT VYRCGPLIDL CRGPHVRHTG KIKALKIHKN SSTYWEGKAD METLQRIYGI SFPDPKMLKE WEKFQEEAKN RDHRKIGRDQ ELYFFHELSP GSCFFLPKGA YIYNALIEFI RSEYRKRGFQ EVVTPNIFNS RLWMTSGHWQ HYSENMFSFE VEKELFALKP MNCPGHCLMF DHRPRSWREL PLRLADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCAME QIEDEIKGCL DFLRTVYSVF GFSFKLNLST RPEKFLGDIE VWDQAEKQLE NSLNEFGEKW ELNSGDGAFY GPKIDIQIKD AIGRYHQCAT IQLDFQLPIR FNLTYVSHDG DDKKRPVIVH RAILGSVERM IAILTENYGG KWPFWLSPRQ VMVVPVGPTC DEYAQKVRQQ FHDAKFMADI DLDPGCTLNK KIRNAQLAQY NFILVVGEKE KISGTVNIRT RDNKVHGERT ISETIERLQQ LKEFRSKQAE EEF