Recombinant humaan Tau 352 (0N3R) /MAPT eiwit

MAPT, also known as TAu, is a neuronal microtubule associated protein found predominantly on axons. The function of this protein is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. MAPT, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Recombinant human MAPT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Microtubule-associated protein isoform 4, fetal tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, G protein beta1/gamma2 subunit-interacting factor 1, protein phosphatase 1 regulatory subunit 103, MTBT1, PPND, FTDP-17, TAU, MSTD, MTBT2, FLJ31424, MGC138549, PPP1R103, tau-40, DDPAC, MAPTL

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

> 85% by SDS-PAGE

38.9 kDa (372aa) confirmed by MALDI-TOF (Real Molecular weight on SDS-PAGE will be shift up)

Tau, Microtubule-associated protein tau, TAu, DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, Microtubule-associated protein tau, ATGP0646-10 µg, ATGP0646-20 µg, ATGP0646-50 µg, ATGP0646-100 µg, ATGP0646-250 µg, ATGP0646-500 µg, ATGP0646-1 mg, ATGP0646-10, ATGP0646-20, ATGP0646-50, ATGP0646-100, ATGP0646-250, ATGP0646-500, ATGP0646-1

Jack CR Jr., et al. (2010) Lancet Neurol. 9 (1) :119-28.; ; Buescher JL., et al. (2010) J Biol Chem. 285 (11) :7957-63.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M Nacl, 1mM DTT, 10% glycerol

Neuroscience

NP_058525

P10636

Human

MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKAEEAGI GDTPSLEDEA AGHVTQARMV SKSKDGTGSD DKKAKGADGK TKIATPRGAA PPGQKGQANA TRIPAKTPPA PKTPPSSGEP PKSGDRSGYS SPGSPGTPGS RSRTPSLPTP PTREPKKVAV VRTPPKSPSS AKSRLQTAPV PMPDLKNVKS KIGSTENLKH QPGGGKVQIV YKPVDLSKVT SKCGSLGNIH HKPGGGQVEV KSEKLDFKDR VQSKIGSLDN ITHVPGGGNK KIETHKLTFR ENAKAKTDHG AEIVYKSPVV SGDTSPRHLS NVSSTGSIDM VDSPQLATLA DEVSASLAKQ GL