Recombinant humaan Tau 412 (1N4R) /MAPT-eiwit

Tau, also known as microtubule-associated protein tau (MAPT), is a protein that stabilizes microtubules. It is abundant in neurons in the central nervous system and is less common elsewhere. When this protein is defective, and no longer stabilizes microtubules property, it can result in dementias, such as Alzheimer's disease. Recombinant human Tau protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Microtubule-associated protein tau isoform 5, tau-E, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, G protein beta1/gamma2 subunit-interacting factor 1, protein phosphatase 1 regulatory subunit 103, MTBT1, PPND, FTDP-17, TAU, MSTD, MTBT2, FLJ31424, MGC138549, PPP1R103, tau-40, DDPAC, MAPTL

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

> 80% by SDS-PAGE

45.1 kDa (432aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Tau, Microtubule-associated protein tau isoform 5, MAPT, MAPTL, MTBT1, TAu, PHF-tau, ATGP1030-10 µg, ATGP1030-20 µg, ATGP1030-50 µg, ATGP1030-100 µg, ATGP1030-250 µg, ATGP1030-500 µg, ATGP1030-1 mg, ATGP1030-10, ATGP1030-20, ATGP1030-50, ATGP1030-100, ATGP1030-250, ATGP1030-500, ATGP1030-1

Cross D., et al. (1993), J Cell Sci. 105 : 51-60.; ; Lubke u., et al. (1994), Am. J. Pathol. 145: 175-188

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Neuroscience

NP_001116539

P10636

Human

MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEAEEAGIG DTPSLEDEAA GHVTQARMVS KSKDGTGSDD KKAKGADGKT KIATPRGAAP PGQKGQANAT RIPAKTPPAP KTPPSSGEPP KSGDRSGYSS PGSPGTPGSR SRTPSLPTPP TREPKKVAVV RTPPKSPSSA KSRLQTAPVP MPDLKNVKSK IGSTENLKHQ PGGGKVQIIN KKLDLSNVQS KCGSKDNIKH VPGGGSVQIV YKPVDLSKVT SKCGSLGNIH HKPGGGQVEV KSEKLDFKDR VQSKIGSLDN ITHVPGGGNK KIETHKLTFR ENAKAKTDHG AEIVYKSPVV SGDTSPRHLS NVSSTGSIDM VDSPQLATLA DEVSASLAKQ GL