Recombinant humaan TAZ/WWTR1-eiwit

WWTR1 is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Recombinant human WWTR1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

WW domain containing transcription regulator 1, TAZ

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

46.5 kDa (423aa)

TAZ, WWTR1, WW domain containing transcription regulator 1, TAZ, ATGP2480-10 µg, ATGP2480-20 µg, ATGP2480-50 µg, ATGP2480-100 µg, ATGP2480-250 µg, ATGP2480-500 µg, ATGP2480-1 mg, ATGP2480-10, ATGP2480-20, ATGP2480-50, ATGP2480-100, ATGP2480-250, ATGP2480-500, ATGP2480-1

Huang, W., et al. (2012) J. Biol. Chem. 287 (31), 26245-26253; ; Pan, J., et al. (2012) Oncol. Rep. 28 (1), 179-185;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Stem cell

NP_001161752

Q9GZV5

Human

MNPASAP PPLPPPGQQV IHVTQDLDTD LEALFNSVMN PKPSSWRKKI LPESFFKEPD SGSHSRQSST DSSGGHPGPR LAGGAQHVRS HSSPASLQLG TGAGAAGSPA QQHAHLRQQS YDVTDELPLP PGWEMTFTAT GQRYFLNHIE KITTWQDPRK AMNQPLNHMN LHPAVSSTPV PQRSMAVSQP NLVMNHQHQQ QMAPSTLSQQ NHPTQNPPAG LMSMPNALTT QQQQQQKLRL QRIQMERERI RMRQEELMRQ EAALCRQLPM EAETLAPVQA AVNPPTMTPD MRSITNNSSD PFLNGGPYHS REQSTDSGLG LGCYSVPTTP EDFLSNVDEM DTGENAGQTP MNINPQQTRF PDFLDCLPGT NVDLGTLESE DLIPLFNDVE SALNKSEPFL TWL