Recombinant humaan TBCB-eiwit

Tubulin folding cofactor B, also known as TBCB, belongs to the Microtubules family. Biosynthesis of functional microtubules involve the participation of several chaperones, termed Tubulin folding cofactors A (TBCA), B (TBCB), D (TBCD), E (TBCE) and C (TBCC), that act on folding intermediates downstream of the cytosolic chaperon, alternatively named TCP. TBCB is a 244 amino acid cytoplasmic protein containing one CAP-Gly domain and in widely expressed. TBCB is involved in the regulation of tubulin heterodimer dissociation and may function as a negative regulator of axonal growth. Recombinant human TBCB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tubulin folding cofactor B, CG22, CKAP1, CKAPI

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29.9 kDa (268aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

TBCB, Tubulin folding cofactor B, CG22, CKAP1, CKAPI, ATGP1581-10 µg, ATGP1581-20 µg, ATGP1581-50 µg, ATGP1581-100 µg, ATGP1581-250 µg, ATGP1581-500 µg, ATGP1581-1 mg, ATGP1581-10, ATGP1581-20, ATGP1581-50, ATGP1581-100, ATGP1581-250, ATGP1581-500, ATGP1581-1

Kortazar D., et al. (2007) Exp Cell Res. 313:425-436.; ; Tian G., et al. (1997) J Cell Biol. 138: 821-832.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol

Heat Shock Proteins

NP_001272

Q99426

Human

MGSSHHHHHH SSGLVPRGSH MGSHMEVTGV SAPTVTVFIS SSLNTFRSEK RYSRSLTIAE FKCKLELLVG SPASCMELEL YGVDDKFYSK LDQEDALLGS YPVDDGCRIH VIDHSGARLG EYEDVSRVEK YTISQEAYDQ RQDTVRSFLK RSKLGRYNEE ERAQQEAEAA QRLAEEKAQA SSIPVGSRCE VRAAGQSPRR GTVMYVGLTD FKPGYWIGVR YDEPLGKNDG SVNGKRYFEC QAKYGAFVKP AVVTVGDFPE EDYGLDEI