Recombinant humaan TBCC-eiwit

TBCC (tubulin folding cofactor C) belongs to the TBCC family. It is plays a role in the regulation of centrosome and Golgi apparatus positioning, with consequences on cell shape and cell migration. Cofactor C is one of four proteins (cofactors A, D, E and C) involved in the pathway leading to correctly folded b-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing b-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/b-tubulin complex; interaction with cofactor C then causes the release of b-tubulin polypeptides. Recombinant human TBCC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tubulin-specific chaperone C, CFC, tubulin folding cofactor C

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

41.7 kDa (369aa) confirmed by MALDI-TOF

TBCC, Tubulin-specific chaperone C, CFC, tubulin folding cofactor C, ATGP1673-10 µg, ATGP1673-20 µg, ATGP1673-50 µg, ATGP1673-100 µg, ATGP1673-250 µg, ATGP1673-500 µg, ATGP1673-1 mg, ATGP1673-10, ATGP1673-20, ATGP1673-50, ATGP1673-100, ATGP1673-250, ATGP1673-500, ATGP1673-1

Goncalves J., et al. (2010) EMBO Rep. 11:194-200; ; Lewis SA, et al. (1996) . J. Cell Biol. 132 (1-2) : 1-4.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Cytoskeleton

NP_003183

Q15814

Human

MESVSCS AAAVRTGDME SQRDLSLVPE RLQRREQERQ LEVERRKQKR QNQEVEKENS HFFVATFARE RAAVEELLER AESVERLEEA ASRLQGLQKL INDSVFFLAA YDLRQGQEAL ARLQAALAER RRGLQPKKRF AFKTRGKDAA SSTKVDAAPG IPPAVESIQD SPLPKKAEGD LGPSWVCGFS NLESQVLEKR ASELHQRDVL LTELSNCTVR LYGNPNTLRL TKAHSCKLLC GPVSTSVFLE DCSDCVLAVA CQQLRIHSTK DTRIFLQVTS RAIVEDCSGI QFAPYTWSYP EIDKDFESSG LDRSKNNWND VDDFNWLARD MASPNWSILP EEERNIQWD