Recombinant humaan TBPL1-eiwit

TBPL1, also known as STuD, TLF, TLP and TRF2, belongs to the TBP family. The TATA box-binding protein (TBP) is an essential component of the basal transcriptional machinery. Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. It is essential for spermiogenesis and believed to be important in expression of developmentally regulated genes. Recombinant human TBPL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

TATA box-binding protein-like protein 1, MGC8389, MGC9620, STuD, TLF, TLP, TRF2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.3 kDa (209aa) confirmed by MALDI-TOF

TBPL1, TATA box-binding protein-like protein 1, MGC8389, MGC9620, STuD, TLF, TLP, TRF2, ATGP1896-10 µg, ATGP1896-20 µg, ATGP1896-50 µg, ATGP1896-100 µg, ATGP1896-250 µg, ATGP1896-500 µg, ATGP1896-1 mg, ATGP1896-10, ATGP1896-20, ATGP1896-50, ATGP1896-100, ATGP1896-250, ATGP1896-500, ATGP1896-1

Ohbayashi T., et al. (1999) Biochem. Biophys. Res. Commun. 255:137-142; ; Rabenstein M.D., et al. (1999) Proc. Natl. Acad. Sci. u.S.A. 96:4791-4796

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 1mM EDTA

Epigenomics (Transcription & Translation)

NP_004856

P62380

Human

MDADSDV ALDILITNVV CVFRTRCHLN LRKIALEGAN VIYKRDVGKV LMKLRKPRIT ATIWSSGKII CTGATSEEEA KFGARRLARS LQKLGFQVIF TDFKVVNVLA VCNMPFEIRL PEFTKNNRPH ASYEPELHPA VCYRIKSLRA TLQIFSTGSI TVTGPNVKAV ATAVEQIYPF VFESRKEIL